MAIN FEATURES
Real time imaging capabilities
Our compact and light-weight FLIM Data Acquisition Card can sustain a continuous stream of data to the software, allowing for real-time imaging reconstruction or spectroscopy analysis. Thanks to a simplified visual interface, the user can swiftly access the desired parameters without the risk of stumbling upon redundant parameter configurations. FLIM LABS always offers prompt customer support in case of need.
Real time FLIM phasor-plot
The virtual absence of any lag between the data detection, acquisition and reconstruction provides a real-time FLIM analysis performed using a phasor-plot approach. The intuitiveness of the phasor-plot analysis lies in its geometrical working principle, which can be summarized using three simple rules: (1) if a cloud of points falls on the semicircle, the fluorescence signal can be described by a single exponential lifetime decay; (2) anything that falls within the semicircle area is a superposition of different fluorescence lifetime values (3) shorter lifetimes progressively distribute along the semicircle in a clockwise manner.
Phasor plot analysis
Our phasor-plot analysis allows the user freedom to choose the appropriate phasor segmentation technique, either by performing simple coloring of the phasor cloud or by applying a color gradient to the entire phasor cloud, highlighting either the phase or the modulation lifetime response. Alternatively, it would be possible to use the entangled circlet options in which a color gradient is stretched across two circlets. For non-experienced users it is also possible to rely on our automated machine learning algorithm, exploiting a GMM (Gaussian Mixture Module) approach which will automatically identify phasor clusters (assigning them colors and ultimately coloring the FLIM image).
- Circlet
- Entangled circlets
- Phase and modulation gradient background
- automatic GMM (Gaussian Mixture Model) – available as optional package
Composite FLIM images
In FLIM Studio, users can visualize the superposition of different lifetime intensity in the FLIM image with unprecedented data rendering and visualization. Different fluorescence lifetime decays can be simultaneously visualized together with local pixel intensity information. This ensures an intuitive representation of the experimental data.
ROI
Starting from your acquired image, you can draw various ROI shapes, including custom outlines to extract the desired details and visualize a particular feature or area in your sample. In this way, you can extract the fluorescence lifetime decay from the selected ROI only and build the corresponding phasor-plot for assessing your experimental results.
Data exporting
Once data has been analyzed, FLIM Studio allows you to export your experiments in the format that best suits your needs. At the moment, the available formats are: MatLab (.mat), Python (.npz), HDF5 (.hdf), .SVG (.svg) for both FLIM-phasor and imaging data. The exported data can be locally stored in your computer and quickly shared with other people by using our social-media instant sharing tools.
Social media result sharing
Available as optional package
FLIM LABS mission is to make FLIM technology and analysis easy and accessible: we want to help building a strong and solid FLIM users community. For this reason, we have created a social-media share package that can be used to instantly share their results via iweb messaging apps (WhatsApp, Telegram), email, or social-media (Linkedin, Twitter). This tool will prove to be useful for sharing results within the team, the online scientific community and/or for seeking tech-help.
In cloud storage
Available as optional package
FLIM LABS mission is to make FLIM technology and analysis easy and accessible: we want to help building a strong and solid FLIM users community. For this reason, we have created a social-media share package that can be used to instantly share their results via iweb messaging apps (WhatsApp, Telegram), email, or social-media (Linkedin, Twitter). This tool will prove to be useful for sharing results within the team, the online scientific community and/or for seeking tech-help.
GIT
FLIM Studio is developed using GIT technologies (
) to keep track of changes and new features implementation. FLIM LABS is committed to supporting and developing FLIM Studio maintenance and new releases.
B2C B2B Bespoke design
For any type of software integration or custom development any part of our software can be customized, as we code routines ourselves. If you need to integrate or develop additional functions, do not hesitate to contact us. We will be happy to tune the software on your needs.
