FLIM stands for Fluorescence Lifetime Imaging. As the name suggests, it is an imaging technique based on the detection of the lifetime of fluorescent molecules present in the sample, rather than their fluorescence emission[…]
The information contained in a FLIM image can be very easy to handle when the phasor analysis approach is introduced. This method allows a graphical representation[…]
Fluorescence Correlation Spectroscopy is a powerful method that allows kinetics and diffusion measurements of fluorescent molecules at a single point inside a sample.
The molecules move in and out of a confocal volume irradiated by a laser[…]
Two-Photon Excitation microscopy is based on the nonlinear optical excitation of a fluorophore induced by a high peak power of ultrafast laser pulses. In contrast to the classical fluorescence microscopy[…]