The information contained in a FLIM image can be very easy to handle when the phasor analysis approach is introduced. This method allows a graphical representation (phasor plot) of the lifetime distribution, making a FLIM image quicker to read. Indeed, the interpretation of the FLIM phasor plot is straightforward and the combination of FLIM with phasors enables the researcher to easily separate different lifetime populations within the same sample.

The possibility to discriminate between different fluorophores with different lifetimes is possible because every species is associated with a precise phasor (the so-called “fingerprint”).